{"id":199,"date":"2015-06-24T08:16:47","date_gmt":"2015-06-24T08:16:47","guid":{"rendered":"http:\/\/mullrich.user.jacobs-university.de\/?page_id=199"},"modified":"2023-11-24T10:23:00","modified_gmt":"2023-11-24T10:23:00","slug":"major-research-interests","status":"publish","type":"page","link":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/curriculum-vitae\/major-research-interests\/","title":{"rendered":"Major Research Interests"},"content":{"rendered":"

\"IMG_0084\"<\/a><\/h2>\n

Molecular analysis of adaptation processes in plant pathogenic bacteria in response to temperature changes:<\/strong><\/p>\n

– differential gene expression in response to temperature changes;
\n– regulation of gene expression in bacteria;
\n– molecular dissection of temperature sensing in bacteria;
\n– in-depth analysis of a model two-component regulatory system;
\n– DNA array technology
\n– changes in membrane fluidity, temperature-responsive incorporation of proteins in the bacterial \u00a0 \u00a0 \u00a0 \u00a0 \u00a0membranes;
\n– general microbial physiology under varying physiological conditions;
\n– temperature-dependent synthesis of secondary metabolites in bacteria;
\n– regulation of export and secretion of proteins in plant pathogens;
\n– exopolysaccharide biosynthesis and function;
\n– comparison of thermo-regulated gene expression in pathogens of animals and plants;
\n– model system: Pseudomonas syringae and the phytotoxin coronatine.<\/p>\n

 <\/p>\n

Molecular and genetic analysis of multi-drug efflux systems in plant-associated bacteria<\/strong>:<\/p>\n

– genetic analysis of multidrug efflux (MDE) systems in Erwinia amylovora
\n– MATE and RND-type efflux pumps and their mode of action
\n– Disposal of plant-borne toxic compounds as a virulence mechanism
\n– Antagonistic activities between E. amylovora and Panthoea agglomerans
\n– Peptide-based antimicrobial compounds and their secretion by bacteria
\n– Biophysical analysis and natural functions of MDE pumps
\n– Analysis of transcriptional regulation of MDE systems, regulatory cascades
\n– Genome-wide coordination of MDE expression<\/p>\n

 <\/p>\n

Molecular interactions of plant pathogenic bacteria with host and non-host plants:<\/strong><\/p>\n

– mechanisms of infection, epiphytic fitness of phytopathogens on plant surfaces;
\n– complete nucleotide sequencing of a 90-kb virulence plasmid from Pseudomonas syringae \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 (identification of pathogenicity islands PAI);
\n– ethylene biosynthesis in phytopathogenic bacteria;
\n– identification of novel virulence factors and mechanisms, respectively;
\n– hypersensitive reaction on non-host plants;
\n– detoxification of plant-borne defense compounds by pathogenic bacteria;
\n– octadecanoid signalling pathways (jasmonic acid), metabolism of octadecanoids;
\n– signal perception and transduction during invasion processes;
\n– early stages of infection, prevention of plant recognition of pathogens.<\/p>\n

 <\/p>\n

Biotechnological applications of thermo-regulated gene expression:<\/strong><\/p>\n

– generation of expression vectors or cassettes for temperature-mediated protein synthesis or \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 overproduction;
\n– detoxification processes (degradation of anti-microbial plant compounds by enzymes of plant \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 pathogens);
\n– epiphytic survival strategies of biocontrol bacteria (UV tolerance, capsule formation, plasmid \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 transfer);
\n– biofilm production and prevention in medical, food-based, and other settings.<\/p>\n

 <\/p>\n

Molecular analysis of marine diatom-bacteria interactions:<\/strong><\/p>\n

– establishing a molecular model system for the investigation of cell-to-cell interactions in marine \u00a0 \u00a0 \u00a0 \u00a0 settings
\n– transparent exopolymeric particles
\n– molecular signaling of interactions between heterotrophic marine bacteria and their \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 photosynthetic eukaryotic hosts<\/p>\n

\"Jacobs<\/a><\/p>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Molecular analysis of adaptation processes in plant pathogenic bacteria in response to temperature changes: – differential gene expression in response to temperature changes; – regulation of gene expression in bacteria; – molecular dissection of temperature sensing in bacteria; – in-depth<\/p>\n","protected":false},"author":12,"featured_media":0,"parent":197,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-199","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/wp-json\/wp\/v2\/pages\/199","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/wp-json\/wp\/v2\/comments?post=199"}],"version-history":[{"count":1,"href":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/wp-json\/wp\/v2\/pages\/199\/revisions"}],"predecessor-version":[{"id":1354,"href":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/wp-json\/wp\/v2\/pages\/199\/revisions\/1354"}],"up":[{"embeddable":true,"href":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/wp-json\/wp\/v2\/pages\/197"}],"wp:attachment":[{"href":"https:\/\/pages.constructor.university\/ullrichmatthiasmicrobiology\/wp-json\/wp\/v2\/media?parent=199"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}